Easy methods for fluorescent staining of Neurospora nuclei.
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چکیده
منابع مشابه
Fluorescent staining of fungal nuclei with a benzimidazol derivative.
A direct staining procedure is described for fluorescence microscopy of fungal nuclei, chromosomes and mitochondria. The fluorochrome is a benzimidazol derivative (33258 Hoechst) known to bind selectively to deoxyribonucleic acid at neutral pH. The advantages of 33258 Hoechst relative to Feulgen compounds used previously to stain these structures include a greater intensity of fluorescence, th...
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In an earlier paper from this laboratory (Gordon, 1958) it was demonstrated that fluorescein-labeled specific antiserum employed as a cellular stain will differentiate Candida albicans and Candida tropicalis from other yeasts and yeastlike organisms, including the remaining members of the genus Candida. The present report describes the preparation and use of a similar reagent for detection and ...
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15 صفحه اولFluorescent Staining Method for Bacterial Endospores
We have developed a quick, simple technique for the fluorescent staining of bacterial endospores. This technique was developed using Bacillus subtilis endospores. Our technique requires minimal sample heating so it can safely be carried out entirely in a glove-box. The endospores stain diagnostically as an oval with a standard fluorescent nucleotide stain, acridine orange.
متن کاملStaining Differences in Cell Nuclei
If the parenchymal cells of the liver of the rat, or certain other cells, are stained with a modified Mallory stain or with iron haematoxylin, the nuclei are stained in two different ways. This effect may be observed after various fixatives. Direct microscopical observation shows that when part of a nucleus is cut away by the microtome-knife, the part of it remaining in the section stains diffe...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1982
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1640